Agrobacterium-Mediated Stable Transformation of Medicinal Plant Andrographis paniculata Callus Expressing β-glucuronidase (GUS) Gene
نویسندگان
چکیده
منابع مشابه
Genetic Engineering of Tomato Plants Expressing β-Glucuronidase through Agrobacterium-mediated Transformation
متن کامل
Agrobacterium-Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene
In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....
متن کاملCharacterization studies on medicinal plant of Andrographis paniculata (NEES)
Andrographis paniculata (Nees), is a valuable traditional medicinal plant and it has many important bioactive compounds. It cures and prevents a number of diseases in human beings. It is a boon of the nature of human healthy life. It cures cold, fever, colic pain, active against inflammatory, antidiabetic activity, antioxidant, antifertility, cardiovascular and anti-virus including inhibited HI...
متن کاملagrobacterium-mediated transformation of pomegranate (punica granatum l.) ‘yousef khani’ using the gus reporter gene
in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....
متن کاملAgrobacterium tumefaciens- mediated Transformation of Plant Cells
Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident ‘tumour inducing’ (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. This bacteria’s capacity for DNA transfer is the basis of most current plant genetic engineering and make it a model system for the study of pathogenic ...
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ژورنال
عنوان ژورنال: Indonesian Journal of Biotechnology
سال: 2015
ISSN: 2089-2241,0853-8654
DOI: 10.22146/ijbiotech.7873